Effects of Fuzheng Huayu recipe on entecavir pharmacokinetics in normal and dimethylnitrosamine-induced hepatic fibrosis rats

Tao, Yang (2018) Effects of Fuzheng Huayu recipe on entecavir pharmacokinetics in normal and dimethylnitrosamine-induced hepatic fibrosis rats. MDPI, 58 (1). pp. 1-953.

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Abstract

Fuzheng Huayu recipe (FZHY) combined with entecavir (ETV) is used to treat the cirrhosis caused by chronic hepatitis B (CHB) infection. Objective: To investigate the effect of FZHY on ETV pharmacokinetics under different conditions. Materials and methods: A model of liver fibrosis was created by intraperitoneal injection of dimethylnitrosamine (DMN; 10 lg/kg) for 4 weeks in Wistar rats. Ultra-high-performance liquid chromatography– tandem mass spectrometry was used to determine the blood concentration of ETV. Pharmacokinetic characteristics of ETV (0.9 mg/kg) were investigated after co-administration with FZHY (0.55 g/kg) at certain time intervals in normal and model rats. Results: The analytical method for ETV was validated at 0.5–50 lg/L with a correlation coefficient ¼ 0.9996, lower limit of quantitation of 0.5 lg/L and mean accuracy of 104.18 ± 9.46%. Compared with the ETV-N group, the pharmacokinetic parameters of the EF-2 group did not change significantly, but that of the EF-0 group decreased in Cmax to 27.38 lg/L, in AUC0–t from 323.84 to 236.67 lg/h/L, and a delay in Tmax from 0.75 to 6.00 h; that of the EF-0 group presented a decrease in Cmax of 61.92%, delay in t1/2 of 2.45 h and delay in Tmax of 2.92 h. The t1/2e and Vd/F of ETV were increased significantly to 8.01 h and 24.38 L/kg in the ETV-M group. Conclusions: The effects of FZHY on ETV pharmacokinetics were diminished with an increase of interval time. The best time to administer both drugs is >2 h apart.

Item Type: Article
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Library of Congress Subject Areas > P Language and Literature > Farmasi
Library of Congress Subject Areas > P Language and Literature > Fakultas Farmasi > Farmasi
Fakultas Farmasi > Farmasi
Depositing User: Unnamed user with email repo@umsb.ac.id
Date Deposited: 28 Sep 2022 03:17
Last Modified: 28 Sep 2022 03:17
URI: http://eprints.umsb.ac.id/id/eprint/479

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