Inhibitory effects of fucoidan on NMDA receptors and l-type Ca2+ channels regulating the Ca2+ responses in rat neurons

Hong, Wu (2019) Inhibitory effects of fucoidan on NMDA receptors and l-type Ca2+ channels regulating the Ca2+ responses in rat neurons. Taylor & Francis Grup, 57 (1). pp. 1-916. ISSN 1388-0209

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Abstract

Fucoidan, a sulphated polysaccharide extracted from brown algae [Fucus vesiculosus Linn. (Fucaceae)], has multiple biological activities. Objective: The effects of fucoidan on Ca2þ responses of rat neurons and its probable mechanisms with focus on glutamate receptors were examined. Materials and methods: The neurons isolated from the cortex and hippocampi of Wistar rats in postnatal day 1 were employed. The intracellular Ca2þ responses triggered by various stimuli were measured in vitro by Fura-2/AM. Fucoidan at 0.5 mg/mL or 1.5 mg/mL was applied for 3 min to determine its effects on Ca2þ responses. RT-PCR was used to determine the mRNA expression of neuron receptors treated with fucoidan at 0.5 mg/mL for 3 h. Results: The Ca2þ responses induced by NMDA were 100% suppressed by fucoidan, and those induced by Bay K8644 90% in the cortical neurons. However, fucoidan has no significant effect on the Ca2þ responses of cortical neurons induced by AMPA or quisqualate. Meanwhile, the Ca2þ responses of hippocampal neurons induced by glutamate, ACPD or adrenaline, showed only a slight decrease following fucoidan treatment. RT-PCR assays of cortical and hippocampal neurons showed that fucoidan treatment significantly decreased the mRNA expression of NMDA-NR1 receptor and the primer pair for L-type Ca2þ channels, PR1/PR2. Discussion and conclusions: Our data indicate that fucoidan suppresses the intracellular Ca2þ responses by selectively inhibiting NMDA receptors in cortical neurons and L-type Ca2þ channels in hippocampal neurons. A wide spectrum of fucoidan binding to cell membrane may be useful for designing a general purpose drug in future.

Item Type:	Article
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Library of Congress Subject Areas > P Language and Literature > Farmasi Library of Congress Subject Areas > P Language and Literature > Fakultas Farmasi > Farmasi Fakultas Farmasi > Farmasi
Depositing User:	Unnamed user with email repo@umsb.ac.id
Date Deposited:	28 Sep 2022 04:13
Last Modified:	28 Sep 2022 04:13
URI:	http://eprints.umsb.ac.id/id/eprint/480

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